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Annual Conference on Bioscience

Berlin, Germany

Basak Kusakci Seker

Basak Kusakci Seker

Eskisehir Osmangazi University, Turkey

Title: Effect of non-thermal atmospheric plasma on surface morphology of radicular dentin: A pilot study

Biography

Biography: Basak Kusakci Seker

Abstract

The purpose of this study was to assess the effect of non-thermal atmospheric plasma-jet on the surface morphology of radicular dentin. Four extracted single-root human teeth were cut 1 mm below the cement-enamel junction in order to obtain samples. The labial sides of the samples were polished and rinsed under deionized water. The plasma stream had a length of 11 mm. The distance between nozzle and dentin was approximately 5 mm. Argon gas is used as carrier gas at a flow of 5 l/min at 2.5 Bar. Roughly 1 cm2 of dentin surface was treated for 30 second, 60 second and 120 second with plasma jet. For SEM observations, the samples were fixed in 2.5% gluteraldehyde in phosphate buffer (pH 7.3) for 24 hours at 4 °C and washed 3 times for 10 minutes each in phosphate buffer. The specimens were then dehydrated in a series of aqueous ethanol solutions with ascending strengths up to 100%. They were dried overnight in a desiccator jar and mounted with silver paint on SEM stubs. Significant differences were detected before and after plasma treat­ment. According to results of this study, it can be assumed that non-thermal atmospheric plasma can significantly alter the morphology of radicular dentin, which is an important characteristic to be considered when plasma is used for clinical applications.