Annual Conference on Bioscience
National Institute of Oceanography and Fisheries (NIOF), Egypt
Title: Molecular identification and biochemical constituent of oleaginous marine microalga Nannochloropsis (Eustigmatophyceae), isolated from Mediterranean Sea, Eastern Harbor of Alexandria coast, Egypt
Biography: Abdel-Wahab Kamil
Screening of local isolated Egyptian marine micro algal species, with potential for aquaculture, biodiesel and biotechnology, are essential to achieve strong micro algal database with successful commercial applications. In this study, identification of Nannochloropsis, isolated from the eastern south of Mediterranean Sea, was carried out using morphological identification, scanning electron microscope and molecular technique (18S rDNA). Moreover, biochemical constituents (lipid, protein, and carbohydrates) were observed. Our promising isolate was carefully identified as Nannochloropsis in the light of obtaining specific genomic band with 982 bp. Alignment of specific band sequence confirmed our identification as Nannochloropsis oceanica strains LAMB0001, N. oceanica strains CCMP531 and N. oceanica strains IMET1 with 100% of identity. On the other hand, total lipid, protein and carbohydrate of isolated and identified N. oceanica strains LAMB0001 were 37.71±4.27%, 22.81±3.71%, and 18.93±2.19% of dry weight, respectively, which make this strain as a good source for either biodiesel production or aquaculture feedstock.