David Pastre
University of Evry, France
Title: Visualization and quantification of protein interactions along microtubules in mammalian cells
Biography
Biography: David Pastre
Abstract
The functions of many proteins and their interplay remain elusive, which limits the developments of diagnostic and treatment of many human diseases. To address this issue, methods are currently developed to decipher protein interactions in cells. We recently developed a new technology to probe protein interactions (PPI) along microtubules in specifically engineered mammalian cells by fluorescence microscopy. A bait protein is brought to microtubules and the presence of putative molecular partners, attracted by the bait protein, is then detected on microtubules by fluorescence microscopy. Here, we present the advantages of this technology compared to other approaches and its latest developments. The domain of applications are broad spanning from discovery of new drugs that target protein or mRNA interactions, identifying molecular targets, exploring the consequences of mutations and the possible corrections of pathogenic consequences.