Sangeeta Yadav
Babasaheb Bhimrao Ambedkar University, India
Title: Detection of quorum sensing molecules during biofilm mediated degradation of lignin
Biography
Biography: Sangeeta Yadav
Abstract
Biofilm mediated bioremediation is a promising technique to treat the complex compounds and biofilm formation is governed by quorum sensing. Lignin is a major component of wood and coloring recalcitrant compound which creates problem during degradation of lignin containing wastewater discharged by the wood based industries like pulp and paper mill industries. A kraft lignin-degrading potential bacterium showed biofilm formation and lignolytic enzyme activity was isolated from sludge of pulp and paper mill and characterized as Enterobacter cancerogenus by 16SrRNA gene sequencing. The E. cancerogenus showed production of name of AHL as quorum sensing molecules and production of EPS which protect the bacteria from adverse condition. However, this strain reduced the color (78%) and lignin content (68%) from kraft lignin-mineral salt medium when supplemented with glucose at pH 7.8 and 30°C after eight days. Functional groups of lignin are degraded effectively by E. cancerogenus proved by Fourier transform infrared spectroscopy, HPLC and gas chromatography-mass spectrometer, and the degradation products were amides, alcohols and acids. Microscopic analysis revealed that biofilms formed in presence of lignin accumulate higher biomass with greater thickness compared to the biofilms produced in presence of glucose as sole carbon source. Meanwhile, the genotoxicity of degraded lignin determined by examining Allium cepa root cell division showed reduced chromosomal aberration. Thus, this study concluded that this strain may be useful for decolorization of industrial wastewater containing high concentration of lignin.