Day 2 :
- Poster Presentations
Location: Foyer
Session Introduction
Abdel-Wahab Kamil
National Institute of Oceanography and Fisheries (NIOF), Egypt
Title: Molecular identification and biochemical constituent of oleaginous marine microalga Nannochloropsis (Eustigmatophyceae), isolated from Mediterranean Sea, Eastern Harbor of Alexandria coast, Egypt
Biography:
Prof. Dr. Abdel-Wahab Kamel is expert in microalgae mass production at the Marine Invertebrates Lab, Aquaculture Division of the National Institute of Oceanography and Fisheries (NIOF). He has thirty years in experience in economic culture media, low cost technique with reduced production cost of different microalgae species. He mainly concern on reduce of production cost of economical algal species, like Spirulina, Dunaliella and Chlorella. He interested in microalgae isolation, optimum growth conditions, mass production and the utilization of microalgae as live food for invertebrates larval feeding. He has earned his B.Sc. degree in 1974 in Agriculture Science, El-Mansoura University; M.Sc., 1985, in the field of Aquatic Plant, El-Zagazig University, and the Ph.D., 1991, on Phytoplankton, El-Zagazig University. He participated as PI, team leader, in national project under the title (The Development Methods of Mass Production of Natural Food, Phytoplankton), funded from NIOF 2015-2016. He participated as Co-PI in national project under the title The (Biodiesel and Protein Concentrate Production from Marine Microalgae), funded from NIOF 2014-2015. He worked in many national project all dealt with microalgae mass production, low cost techniques. He is a supervisor on many theses in Egypt and published a valuable scientific paper.
Abstract:
Screening of local isolated Egyptian marine micro algal species, with potential for aquaculture, biodiesel and biotechnology, are essential to achieve strong micro algal database with successful commercial applications. In this study, identification of Nannochloropsis, isolated from the eastern south of Mediterranean Sea, was carried out using morphological identification, scanning electron microscope and molecular technique (18S rDNA). Moreover, biochemical constituents (lipid, protein, and carbohydrates) were observed. Our promising isolate was carefully identified as Nannochloropsis in the light of obtaining specific genomic band with 982 bp. Alignment of specific band sequence confirmed our identification as Nannochloropsis oceanica strains LAMB0001, N. oceanica strains CCMP531 and N. oceanica strains IMET1 with 100% of identity. On the other hand, total lipid, protein and carbohydrate of isolated and identified N. oceanica strains LAMB0001 were 37.71±4.27%, 22.81±3.71%, and 18.93±2.19% of dry weight, respectively, which make this strain as a good source for either biodiesel production or aquaculture feedstock.
Emre Seker
Eskisehir Osmangazi University, Turkey
Title: Surface treatment of PMMA with argon plasma-jet: A pilot study
Biography:
Emre Seker has completed his undergraduate education and PhD from Ankara University and Near East University respectively. He is as a Lecturer and Clinical Specialist at Eskisehir Osmangazi University, Faculty of Dentistry, Department of Prosthodontics as an Assistant Professor. He has published more than 30 papers and presentations and continues to study on surface treatment techniques of dental materials, CADCAM dentistry and plasma technology.
Abstract:
This study aimed to evaluate the surface changes and wettability of PMMA as a prosthetic material after different durations of argon plasma-jet surface treatment. Four Poly Methyl Methacrylate (PMMA) discs were machined and smoothed with silicon polishing discs. The surface changes were evaluated in a control group and in groups with different plasma-jet exposure application times [30-60-120 seconds (s)]. The average contact angle (CA) measurements and surface changes were recorded via a tensiometer and scanning electron microscope (SEM) respectively. According to the results, the argon plasma-jet surface treatment markedly affects the wettability properties. With an increase in the application time, a remarkable reduction in CA and surface changes were observed. Within the limitation of this study it can be concluded that the argon plasma-jet could enhance the wetting performance and adhesive capability of PMMA.
Jorge Enrique Lozano
National University of Bahia Blanca, Argentina
Title: Yeast biofilm development and rheological properties development on turbulent flow regimes
Biography:
Jorge Enrique Lozano has completed his PhD in Chemical Engineering in 1988. He is a full Professor in Food Engineering and a Member of the National Science and Technology Council of Argentina. He published more than 80 papers in reputed journals, two books and several chapters on food science and technology. He also serves as an Editorial Board Member of the Journal of Food Process Engineering (since 1998) and the Food Engineering Reviews (Since 2008).
Abstract:
In food processing lines or in complex equipment, microorganisms are exposed to varying hydrodynamic conditions caused by the flow of liquid food and biofilms grown under a wide distribution of hydrodynamic strengths. Using industrially relevant strains of yeasts, it was demonstrated that biofilms formed on stainless steel at Reynolds (Re) numbers ranging from 294,000 to 1.2×106. These growth phases transform adherent blastospores to well-defined cellular communities encased in an extracellular matrix and biofilm formation increases when increasing Re number and time. In situ rheological behavior of yeast biofilms growing on stainless steel under turbulent flow was also investigated. The species used (R. mucilaginosa, C. krusei, C. kefyr and C. tropicalis) were isolated from a clarified apple juice industry. The flow conditions impacted biofilm composition over time with a predominance of C. krusei under static and turbulent flow. Structural variations occurred with a tighter appearance under dynamic flow. Under turbulent flow there was an increase of 112 μm in biofilm thickness and cell morphology was governed by hyphal structures and rounded cells. Yeast biofilms were determined to be viscoelastic materials with a predominantly solid-like behavior and rheological values were not significantly affected by the flow conditions or the growth time. At large deformations their weak structure collapsed beyond a critical strain. The present work could represent a starting point for developing in situ measurements of yeast rheology and contribute to a thin body of knowledge about fungal biofilm formation.
Kholood Mohamed Gharieb
Zagazig University, Egypt
Title: Isolation and characterization of new bacteriophages infecting Ralstonia solanacearum in Egypt
Biography:
Kholood Mohamed Gharieb is a Demonstrator at the Faculty of Science, Zagazig University, Egypt. She is also a Master’s degree student conducting research on Ralstonia solanacearum bacterium and wilt diseases in Egypt.
Abstract:
Ralstonia solanacearum is a Gram-negative bacterium and the causative agent of bacterial wilt in many important crops. The bacteria were isolated from infected potato tubers and Egyptian soil. The isolates were characterized by CPG medium. In this study three different bacteriophages infecting the phytopathogen Ralstonia solanacearum were isolated from Egyptian soil. The phages showed different host ranges when tested against different R. solanacearum strains. These phages were characterized as two myoviruses and one siphovirus with very non-contractile tail based on their morphology under electron microscope. These phages showed diverged genomic sequences revealed by restriction enzyme digestion analysis. The isolated phages in this study represent valuable resources with potential uses in biocontrol of bacterial wilt in Egypt.