Dennis M Lox
Sports and Regenerative Medicine Centers, USA
Title: Bio-scaffolds and stem cells in knees
Biography
Dennis M Lox is a world renowned Sports and Regenerative Medicine Expert. He has lectured internationally with some of the most acclaimed Regenerative Medicine and Stem Cell researchers in the world. He has a special interest in Regenerative and Stem Cell Medicine as it relates to athletes, the aging population, osteoarthritis, and Avascular Necrosis (AVN). He has edited two medical textbooks, eight medical textbook chapters, authored numerous scientific articles and abstracts. He continues to maintain an active sport and regenerative medicine practice in Beverly Hills, California and Tampa Bay, Florida region. He has treated patients from around the globe. These diverse patients include professional athletes to patients in their nineties. Utilizing this experience, he incorporates an individualized approach to each patient.
Abstract
The use of various scaffolds, matrix materials, and stem cells in regenerative medicine and tissue engineering has proliferated in the last decade. The inter-lapping nature of regenerative medicine and tissue engineering, has explored the fundamentals of bioscience, cellular signaling, and aging. The various engineered scaffolds have included synthetic, allogenic, autologous, xenographic, natural matrix substrates and 3-D bio-printing. Cellular medicine has also explored various types of cells including autologous, allogenic, and perinatal. The animal model has yielded promising results, yet a fundamental difference in human diverse behavior and inter-patient variability, may ultimately prove impossible to extrapolate from more simplistic animal model studies. The inherent nature of degenerative osteoarthritis of the knee is a progressive phenomenon. The role of cellular medicine and bio-scaffolds may alter the nature of osteoarthritis over time, thus impacting functional level and quality of life (QoL). The future of improving quality of life (QoL) may find a common thread in preventative and regeneration medicine.
Shorayeva Kamshat Abitkhanovna
Research Institute for Biological Safety Problems, Kazakhstan
Title: Diagnosis of viral diseases in birds with oligonucleotide microarray
Biography
Shorayeva Kamshat Abitkhanovna is a Researcher of Molecular Biology and Genetic Engineering at Research Institute for Biological Safety Problems. She is a Specialist in the field of Molecular Biology, Biochemistry, Biotechnology and Diagnostics of Viral Infections. She is involved in performing international and national research projects and has more than 30 publications in scientific journals.
Abstract
Diversity of avian viral diseases impedes their diagnosis by traditional research methods. To solve the problem, we developed a new oligonucleotide microchip for rapid simultaneous diagnosis of viral diseases in birds such as avian influenza (AI), Newcastle disease (ND), infectious bronchitis (IB) and infectious bursal disease (IBD). The designed oligonucleotide microchip consists of 16 identical subarrays for simultaneous rapid detection of avian viruses: AIV, NDV, IBV and IBDV in single- and mixed-virus infections. Probes for the proposed microarray are designed on the basis of conserved regions from gene fragments encoding the major viral proteins: NP and M (AIV), NP (VBI), VP2 (VIBB) and S1 (VIB), provided in GenBank database. Versatility of test makes it suitable for a wide use in veterinary laboratories for rapid detection of avian infections. So, the developed microarray for rapid diagnosis of avian viral diseases can be used in mass analysis in the system of routine epidemiological surveillance owing to its ability to test one sample for simultaneous detection of AIV, NDV, IBV and IBDV in cases of single and mixed viral infections. At the same time, duration of the analysis decreases dozen times versus classical methods and the proposed scheme of specimen preparation allows conducting assays immediately in small veterinary laboratories thus avoiding transportation of thermolabile RNA.